pgl3 luciferase vectors (Promega)
90
Structured Review
Promega
pgl3 luciferase vectors
Pgl3 Luciferase Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+luciferase+vectors/pgl3+basic/pm40664766-81-25-28
Average 90 stars, based on 1 article reviews
Pgl3 Luciferase Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+luciferase+vectors/pgl3+basic/pm40664766-81-25-28
Average 90 stars, based on 1 article reviews
pgl3 luciferase vectors - by Bioz Stars,
2026-10
90/100 stars
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Binding Assay:Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR‐1236/HER2 signaling pathway Article Snippet: .. The fragments containing the predicted binding sites for miR‐1236 in circ0030018 sequence and HER2 3’‐UTR were synthesized and inserted to the Article Title: CircRNA ZFR promotes cell progression by regulating miR-96a-5p/SLC1A1 axis in hepatocellular carcinoma. Article Snippet: The blots were then incubated with HRP secondary antibodies (Dilution: 1: 6000, Aspen, China) for 2 h at room temperature and the bands visualized using enhanced chemiluminescence detection system and a Chemidoc XRS imager system (Bio-Rad Laboratories, USA). .. The wild-type (WT) or mutated (MUT) circRNA ZFR or 30-untranslated region (30-UTR) region of SLC1A1 containing miR-96a-5p specific binding sites were amplified and sub-cloned into Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR-1236/HER2 signaling pathway. Article Snippet: .. The fragments containing the predicted binding sites for miR-1236 in circ0030018 sequence and HER2 3’-UTR were synthesized and inserted to the Sequencing:Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR‐1236/HER2 signaling pathway Article Snippet: .. The fragments containing the predicted binding sites for miR‐1236 in circ0030018 sequence and HER2 3’‐UTR were synthesized and inserted to the Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR-1236/HER2 signaling pathway. Article Snippet: .. The fragments containing the predicted binding sites for miR-1236 in circ0030018 sequence and HER2 3’-UTR were synthesized and inserted to the Synthesized:Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR‐1236/HER2 signaling pathway Article Snippet: .. The fragments containing the predicted binding sites for miR‐1236 in circ0030018 sequence and HER2 3’‐UTR were synthesized and inserted to the Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR-1236/HER2 signaling pathway. Article Snippet: .. The fragments containing the predicted binding sites for miR-1236 in circ0030018 sequence and HER2 3’-UTR were synthesized and inserted to the Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Caspase-3 activity assay Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. Luciferase reporter assay The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Luciferase:Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR‐1236/HER2 signaling pathway Article Snippet: .. The fragments containing the predicted binding sites for miR‐1236 in circ0030018 sequence and HER2 3’‐UTR were synthesized and inserted to the Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Article Title: CircRNA ZFR promotes cell progression by regulating miR-96a-5p/SLC1A1 axis in hepatocellular carcinoma. Article Snippet: The blots were then incubated with HRP secondary antibodies (Dilution: 1: 6000, Aspen, China) for 2 h at room temperature and the bands visualized using enhanced chemiluminescence detection system and a Chemidoc XRS imager system (Bio-Rad Laboratories, USA). .. The wild-type (WT) or mutated (MUT) circRNA ZFR or 30-untranslated region (30-UTR) region of SLC1A1 containing miR-96a-5p specific binding sites were amplified and sub-cloned into Article Title: Overexpression of LINC00607 inhibits cell growth and aggressiveness by regulating the miR-1289/EFNA5 axis in non-small-cell lung cancer Article Snippet: Cell lysates and biotinylated RNAs were incubated with streptavidin-coated magnetic beads (Life Technologies) at 4°C for 3 h. Finally, RNA–protein binding mixture was purified and then detected by RT-qPCR. .. The wild-type (WT) LINC00607 and 3′-untranslated regions of WT EFNA5 were subcloned into Article Title: miR-146a-5p regulates autophagy and NLRP3 inflammasome activation in epithelial barrier damage in the in vitro cell model of ulcerative colitis through the RNF8/Notch1/mTORC1 pathway. Article Snippet: Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon that can be influenced by microRNAs (miRNAs).. This study aims to investigate the impact of miR-146a-5p on lipopolysaccharide (LPS)-induced Caco2/HT-29 cell autophagy and NLRP3 inflammasome activation and the underlying mechanism, with the aim of identifying potential therapeutic targets.. We used LPS to establish Caco-2/HT-29 cell models and measured cell viability by CCK-8. Article Title: Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145-5p. Article Snippet: Objective: Stem cell/exosome therapy is a novel strategy for primary ovarian insufficiency (POI).. This paper is to examine the role of human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) Article Title: Isoliquiritigenin inhibits circ0030018 to suppress glioma tumorigenesis via the miR-1236/HER2 signaling pathway. Article Snippet: .. The fragments containing the predicted binding sites for miR-1236 in circ0030018 sequence and HER2 3’-UTR were synthesized and inserted to the Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Caspase-3 activity assay Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. Luciferase reporter assay The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Mutagenesis:Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Article Title: miR-146a-5p regulates autophagy and NLRP3 inflammasome activation in epithelial barrier damage in the in vitro cell model of ulcerative colitis through the RNF8/Notch1/mTORC1 pathway. Article Snippet: Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon that can be influenced by microRNAs (miRNAs).. This study aims to investigate the impact of miR-146a-5p on lipopolysaccharide (LPS)-induced Caco2/HT-29 cell autophagy and NLRP3 inflammasome activation and the underlying mechanism, with the aim of identifying potential therapeutic targets.. We used LPS to establish Caco-2/HT-29 cell models and measured cell viability by CCK-8. Article Title: Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145-5p. Article Snippet: Objective: Stem cell/exosome therapy is a novel strategy for primary ovarian insufficiency (POI).. This paper is to examine the role of human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Caspase-3 activity assay Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. Luciferase reporter assay The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Clone Assay:Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Article Title: miR-146a-5p regulates autophagy and NLRP3 inflammasome activation in epithelial barrier damage in the in vitro cell model of ulcerative colitis through the RNF8/Notch1/mTORC1 pathway. Article Snippet: Ulcerative colitis (UC) is a chronic inflammatory disease affecting the colon that can be influenced by microRNAs (miRNAs).. This study aims to investigate the impact of miR-146a-5p on lipopolysaccharide (LPS)-induced Caco2/HT-29 cell autophagy and NLRP3 inflammasome activation and the underlying mechanism, with the aim of identifying potential therapeutic targets.. We used LPS to establish Caco-2/HT-29 cell models and measured cell viability by CCK-8. Article Title: Human umbilical cord mesenchymal stem cell-derived extracellular vesicles improve ovarian function in rats with primary ovarian insufficiency by carrying miR-145-5p. Article Snippet: Objective: Stem cell/exosome therapy is a novel strategy for primary ovarian insufficiency (POI).. This paper is to examine the role of human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUCMSC-EVs) Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Caspase-3 activity assay Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. Luciferase reporter assay The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into Amplification:Article Title: CircRNA ZFR promotes cell progression by regulating miR-96a-5p/SLC1A1 axis in hepatocellular carcinoma. Article Snippet: The blots were then incubated with HRP secondary antibodies (Dilution: 1: 6000, Aspen, China) for 2 h at room temperature and the bands visualized using enhanced chemiluminescence detection system and a Chemidoc XRS imager system (Bio-Rad Laboratories, USA). .. The wild-type (WT) or mutated (MUT) circRNA ZFR or 30-untranslated region (30-UTR) region of SLC1A1 containing miR-96a-5p specific binding sites were amplified and sub-cloned into Reporter Assay:Article Title: miR-29c-3p Attenuates β-Amyloid-Induced Neurotoxicity in Alzheimer’s Disease Through Regulating β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Article Snippet: Caspase-3 activity assay Transfected PC12 cells were treated with 10 μM Aβ for 24 h. The absorbance was measured at 405nm using a microplate reader (Becton Dickinson, Mountain View, CA, USA) using the caspase-3 assay Kit (Solarbio, Beijing, China). .. Luciferase reporter assay The wild type or mutant-type 3′-UTR of BACE1 sequences containing putative miR-29c-3p-binding sites were synthesized and cloned into |